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Abstract

Cardiovascular disease conditions, such as myocardial infarction (MI), are prominent contributors to global mortality. Isoprenaline induces acute myocardial damage and infarction. Polyalthia longifolia (PL), has been reported to possess various potential health benefits. Thus, this study evaluated the protective role of the phenol-rich fraction of PL leaf on isoprenaline (ISO)-mediated myocardial infarction in rats. Forty male Wistar rats (265±15g) were randomly and equally grouped into five (n=8). Group A (control) received I mL/kg distilled water, B: ISO at 90 mg/kg, C: vitamin C (Vit C) at 100 mg/kg and ISO, and D and E: PL at 100 mg/kg and 200 mg/kg, respectively and ISO. Treatment was done orally and lasted for 13 consecutive days except for ISO which was given subcutaneously on the 13th day. Blood pressure was monitored following acclimatisation. The whole blood and the heart tissue were collected and analysed for antioxidants, inflammation, oxidative stress markers, and histopathology. Isoprenaline increased blood pressure parameters in group B. These parameters were reversed in Vit-C and PL-exposed groups. Isoprenaline significantly (p<0.05) induced oxidative stress and inflammation, and reduced antioxidant markers in group B. Vit C and PLameliorated the isoprenaline-induced toxicity in groups C, D, and E. ISO induced inflammatory cells, infarction, and oedema in the heart tissue in group B. These changes were mildly reversed in Vit-C and PL-treated rats. In conclusion, Polyalthia longifolia phenol-rich fraction mitigated isoprenaline-induced myocardial toxicity in rats, demonstrating effects comparable to those of Vitamin C.

Keywords

Polyalthia longifolia Isoproterenol Cardiovascular diseases Oxidative stress Antioxidants

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Polyalthia Longifolia Ameliorates Isoprenaline-Induced Myocardial Toxicity via Markers of Oxidative Stress and Inflammation in Wistar Rats. (2025). Sahel Journal of Veterinary Sciences, 22(3), 14-22. https://doi.org/10.54058/vkxwp365
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Polyalthia Longifolia Ameliorates Isoprenaline-Induced Myocardial Toxicity via Markers of Oxidative Stress and Inflammation in Wistar Rats. (2025). Sahel Journal of Veterinary Sciences, 22(3), 14-22. https://doi.org/10.54058/vkxwp365

References

  1. Akila, P. and Vennila, L. (2016). Chlorogenic acid a dietary polyphenol attenuates isoproterenol induced myocardial oxidative stress in rat myocardium: An in vivo study. Biomedicine &Pharmacotherapy 84: 208–214. https://doi.org/10.1016/j.biopha.2016.09.028
  2. Avwioro, O.G. (2002). Histochemistry and tissue pathology. Ibadan: Claverianum Press. 134-213.
  3. Beutler, E., Duron, O. and Kelly, B. M. (1963). Improved method for the determination of blood glutathione. The Journal of Laboratory and Clinical Medicine, 61: 882–888.
  4. Boersma, E., Mercado, N., Poldermans, D., Gardien, M., Vos, J. and Simoons, M. L. (2003). Acute myocardial infarction. Lancet (London, England), 361(9360): 847–858. https://doi.org/10.1016/S0140-6736(03)12712-2
  5. Chen, C. Y., Chang, F. R., Shih, Y. C., Hsieh, T. J., Chia, Y. C., Tseng, H. Y., Chen, H. C., Chen, S. J., Hsu, M. C. and Wu, Y. C. (2000). Cytotoxic constituents of Polyalthia longifolia var. pendula. Journal of Natural Products, 63(11): 1475–1478. https://doi.org/10.1021/np000176e.
  6. Faizi, S., Khan, R. A., Mughal, N. R., Malik, M. S., Sajjadi, K. E. and Ahmad, A. (2008). Antimicrobial activity of various parts of Polyalthia longifolia var. pendula: isolation of active principles from the leaves and the berries. Phytotherapy Research: PTR, 22(7): 907–912. https://doi.org/10.1002/ptr.2414.
  7. Habig, W. H., Pabst, M. J. and Jakoby, W. B. (1974). Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. The Journal of Biological Chemistry, 249(22): 7130–7139.
  8. Hall, I. P., Donaldson, J. and Hill, S. J. (1989). Inhibition of histamine-stimulated inositol phospholipid hydrolysis by agents which increase cyclic AMP levels in bovine tracheal smooth muscle. British journal of Pharmacology, 97(2): 603–613. https://doi.org/10.1111/j.1476-5381.1989.tb11992.x.
  9. Hareeri, R. H., Alam, A. M., Bagher, A. M., Alamoudi, A. J., Aldurdunji, M. M., Shaik, R. A., Eid, B. G. and Ashour, O. M. (2023). Protective Effects of 2-Methoxyestradiol on Acute Isoproterenol-Induced Cardiac Injury in Rats. Saudi Pharmaceutical Journal: SPJ: the official publication of the Saudi Pharmaceutical Society, 31(10): 101787. https://doi.org/10.1016/j.jsps.2023.101787
  10. Hertog, M. G., Feskens, E. J., Hollman, P. C., Katan, M. B. and Kromhout, D. (1993). Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study. Lancet (London, England), 342(8878): 1007–1011. https://doi.org/10.1016/0140-6736(93)92876-u.
  11. Hosseini, A., Rajabian, A., Sobhanifar, M. A., Alavi, M. S., Taghipour, Z., Hasanpour, M., Iranshahi, M., Boroumand-Noughabi, S., Banach, M. and Sahebkar, A. (2022). Attenuation of isoprenaline-induced myocardial infarction by Rheum turkestanicum. Biomedicine &Pharmacotherapy 148: 112775. https://doi.org/10.1016/j.biopha.2022.112775.
  12. Jossang, A., Leboeuf, M. and Cave, A. (1982). Un nouveau type d’alcaloïdesisoquinoleiques, les bisaporphines. Tetrahedron Lett. 23: 5147
  13. Katkar, K. V., Suthar, A. C. and Chauhan, V. S. (2010). The chemistry, pharmacologic, and therapeutic applications of Polyalthia longifolia. Pharmacognosy Reviews, 4(7): 62–68. https://doi.org/10.4103/0973-7847.65329
  14. Kayali, R., Cakatay, U., Akçay, T. and Altuğ, T. (2006). Effect of alpha-lipoic acid supplementation on markers of protein oxidation in post-mitotic tissues of ageing rat. Cell Biochemistry and Function, 24(1): 79–85.https://doi.org/10.1002/cbf.1190.
  15. Kloner R. A. (2006). Natural and unnatural triggers of myocardial infarction. Progress in Cardiovascular Diseases, 48(4): 285–300. https://doi.org/10.1016/j.pcad.2005.07.001.
  16. Krijnen, P. A., Nijmeijer, R., Meijer, C. J., Visser, C. A., Hack, C. E. and Niessen, H. W. (2002). Apoptosis in myocardial ischaemia and infarction. Journal of Clinical Pathology, 55(11): 801–811. https://doi.org/10.1136/jcp.55.11.801.
  17. Lee, Y. and Gustafsson, A. B. (2009). Role of apoptosis in cardiovascular disease. Apoptosis: An International Journal on Programmed Cell Death, 14(4): 536–548. https://doi.org/10.1007/s10495-008-0302-x.
  18. Levine, R. L., Garland, D., Oliver, C. N., Amici, A., Climent, I., Lenz, A. G., Ahn, B. W., Shaltiel, S. and Stadtman, E. R. (1990). Determination of carbonyl content in oxidatively modified proteins. Methods in Enzymology, 186: 464–478. https://doi.org/10.1016/0076-6879(90)86141-h
  19. Manjunatha, S., Shaik, A. H., Maruthi, P. E., SulimanYousef, O., Altaf, M. and Lakshmi, D. K. (2020). Combined cardio-protective ability of syringic acid and resveratrol against isoproterenol induced cardio-toxicity in rats via attenuating NF-kB and TNF-α pathways.Scientific Reports 10: 3426. https://doi.org/10.1038/s41598-020-59925-0.
  20. Meeran, M. F. N., Jagadeesh, G. S. and Selvaraj, P. (2015). Catecholamine toxicity triggers myocardial membrane destabilization in rats: thymol and its counter action. RSC Advances, 5: 43338–43344.
  21. Misra, H. P. and Fridovich, I. (1972). The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. The Journal of Biological chemistry, 247(10): 3170–3175.
  22. Mnafgui, K., Hajji, R., Derbali, F., Khlif, I., Kraiem, F., Ellefi, H., Elfeki, A., Allouche, N. and Gharsallah, N. (2016). Protective Effect of Hydroxytyrosol Against Cardiac Remodeling After Isoproterenol-Induced Myocardial Infarction in Rat. Cardiovascular Toxicology, 16(2): 147–155. https://doi.org/10.1007/s12012-015-9323-1.
  23. Olaleye, S. B., Adaramoye, O. A., Erigbali, P. P. and Adeniyi, O. S. (2007). Lead exposure increases oxidative stress in the gastric mucosa of HCl/ethanol-exposed rats. World Journal of Gastroenterology, 13(38): 5121–5126. https://doi.org/10.3748/wjg.v13.i38.5121.
  24. Oyagbemi, A. A., Omobowale, T. O., Akinrinde, A. S., Saba, A. B., Ogunpolu, B. S. and Daramola, O. (2015). Lack of reversal of oxidative damage in renal tissues of lead acetate-treated rats. Environmental Toxicology, 30(11): 1235–1243. https://doi.org/10.1002/tox.21994.
  25. Oyagbemi, A. A., Omobowale, T. O., Asenuga, E. R., Adejumobi, A. O., Ajibade, T. O., Ige, T. M., Ogunpolu, B. S., Adedapo, A. A. and Yakubu, M. A. (2017). Sodium fluoride induces hypertension and cardiac complications through generation of reactive oxygen species and activation of nuclear factor kappa beta. Environmental Toxicology, 32(4): 1089–1101. https://doi.org/10.1002/tox.22306.
  26. Panda, S., Kar, A. and Biswas, S. (2017). Preventive effect of Agnucastoside C against Isoproterenol-induced myocardial injury. Scientific Reports, 7(1): 16146. https://doi.org/10.1038/s41598-017-16075-0.
  27. Priscilla, D. H. and Prince, P. S. (2009). Cardioprotective effect of gallic acid on cardiac troponin-T, cardiac marker enzymes, lipid peroxidation products and antioxidants in experimentally induced myocardial infarction in Wistar rats. Chemico-biological Interactions, 179(2-3): 118–124. https://doi.org/10.1016/j.cbi.2008.12.012.
  28. Qin, M., Liu, T., Hu, H., Wang, T., Yu, S. and Huang, C. (2013). Effect of isoprenaline chronic stimulation on APD restitution and ventricular arrhythmogenesis. Journal of Cardiology. 61(2): 162–168. https://doi.org/10.1016/j.jjcc.2012.08.016
  29. Raghunathan, K. and Mitra, R. (1985). Pharmacognosy of Indigenous Drugs, Vol. 1, Central Council for Research in Ayurveda and Siddha, New Delhi. 127-139.
  30. Raish, M. (2017). Momordica charantia polysaccharides ameliorate oxidative stress, hyperlipidemia, inflammation, and apoptosis during myocardial infarction by inhibiting the NF-κBsignaling pathway. International Journal of Biological Macromolecules, 97: 544–551. https://doi.org/10.1016/j.ijbiomac.2017.01.074.
  31. Rajadurai, M. and Stanely M.P. P. (2007). Preventive effect of naringin on cardiac markers, electrocardiographic patterns and lysosomal hydrolases in normal and isoproterenol-induced myocardial infarction in Wistar rats. Toxicology, 230(2-3): 178–188. https://doi.org/10.1016/j.tox.2006.11.053.
  32. Ramic-Catak A, Mesihović-Dinarevic S., andPrnjavorac, B. (2023). Public Health Dimensions of CVD Prevention and Control - Global Perspectives and Current Situation in the Federation of BiH. Materia socio-medica.35(2): 88–93. https://doi.org/10.5455/msm.2023.35.88-93
  33. Ravikumar, Y. S., Mahadevan, K. M., Kumaraswamy, M. N., Vaidya, V. P., Manjunatha, H., Kumar, V. and Satyanarayana, N.D. (2008). Antioxidant, cytotoxic and genotoxic evaluation of alcoholic extract of Polyalthiacerasoides (Roxb.) Bedd. Environmental Toxicology and Pharmacology, 26: 142-146.
  34. Ravikumar, Y. S., Mahadevan, K. M., Manjunatha, H. and Satyanarayana, N.D. (2010). Antiproliferative, apoptotic and antimutagenic activity of isolated compounds from Polyalthiacerasoides seeds. Phytomedicine. 17: 513-518
  35. Sastri, B. N. (1969). The wealth of India Raw Materials, Information and Publication Directorate. CSIR, New Delhi. 3: 101.
  36. Schömig, A. (1990). Catecholamines in myocardial ischemia. Systemic and cardiac release. Circulation, 82(3 Suppl): II13–II22.
  37. Shahzad, S., Mateen, S., Naeem, S. S., Akhtar, K., Rizvi, W. and Moin, S. (2019). Syringic acid protects from isoproterenol induced cardiotoxicity in rats. European Journal of Pharmacology. 849: 135-145,
  38. https://doi. org/10.1016/j.ejphar.2019.01.056.
  39. Shaikh, S., Bhatt, L. K. and Barve, K. (2019). Attenuation of isoproterenol-induced cardiotoxicity in rats by Narirutin rich fraction from grape fruit. Phytomedicine: International Journal of Phytotherapy and Phytopharmacology, 55: 222–228. https://doi.org/10.1016/j.phymed.2018.06.037.
  40. Sharma, S., Khan, V., Dhyani, N., Najmi, A. K. and Haque, S. E. (2020). Icariin attenuates isoproterenol-induced cardiac toxicity in Wistar rats via modulating cGMP level and NF-κBsignaling cascade. Human & Experimental Toxicology, 39(2): 117–126. https://doi.org/10.1177/0960327119890826.
  41. Son, T. G., Camandola, S. and Mattson, M. P. (2008). Hormetic dietary phytochemicals. Neuromolecular Medicine, 10(4): 236–246. https://doi.org/10.1007/s12017-008-8037-y.
  42. Song, Y. B., Hahn, J. Y., Gwon, H. C., Kim, J. H., Lee, S. H., Jeong, M. H. and KAMIR investigators (2008). The impact of initial treatment delay using primary angioplasty on mortality among patients with acute myocardial infarction: from the Korea acute myocardial infarction registry. Journal of Korean Medical Science, 23(3): 357–364. https://doi.org/10.3346/jkms.2008.23.3.357.
  43. Tang, Y. N., He, X. C., Ye, M., Huang, H., Chen, H. L., Peng, W. L., Zhao, Z. Z., Yi, T. and Chen, H. B. (2015). Cardioprotective effect of total saponins from three medicinal species of Dioscorea against isoprenaline-induced myocardial ischemia. J Ethnopharmacology 4: 175:451-5. doi: 10.1016/j.jep.2015.10.004.
  44. Tanna, A., Nair, R. and Chanda, S. (2009). Assessment of anti-inflammatory and hepatoprotective potency of Polyalthia longifolia var. pendula leaf in Wistar albino rats. Journal of Natural Medicines, 63(1): 80–85. https://doi.org/10.1007/s11418-008-0288-2.
  45. Terentyev, D., Györke, I., Belevych, A. E., Terentyeva, R., Sridhar, A., Nishijima, Y., de Blanco, E. C., Khanna, S., Sen, C. K., Cardounel, A. J., Carnes, C. A. and Györke, S. (2008). Redox modification of ryanodine receptors contributes to sarcoplasmic reticulum Ca2+ leak in chronic heart failure. Circulation Research, 103(12): 1466–1472. https://doi.org/10.1161/CIRCRESAHA.108.184457.
  46. Troudi, A., Soudani, N., Amara, I. B., Bouaziz, H., Ayadi, F. M., &Zeghal, N. (2012). Oxidative damage in erythrocytes of adult rats and their suckling pups exposed to gibberellic acid. Toxicology and Industrial Health, 28(9), 820–830. https://doi.org/10.1177/0748233711425068
  47. Vaduganathan, M., Mensah, G. A., Turco, J. V., Fuster, V. and Roth, G. A. (2022). The Global Burden of Cardiovascular Diseases and Risk: A Compass for Future Health. Journal of the American College of Cardiology, 80(25): 2361–2371. https://doi.org/10.1016/j.jacc.2022.11.005.
  48. Varshney, R. and Kale, R. K. (1990). Effects of calmodulin antagonists on radiation-induced lipid peroxidation in microsomes. International Journal of Radiation Biology, 58(5): 733–743. https://doi.org/10.1080/09553009014552121
  49. Wan, Y., Liu, J., Mai, Y., Hong, Y., Jia, Z., Tian, G., Liu, Y., Liang, H. and Liu, J. (2024). Current advances and future trends of hormesis in disease. NPJ Aging. 10(1)” 26. doi: 10.1038/s41514-024-00155-3.
  50. Wang, Y., Branicky, R., Noë, A. and Hekimi, S. (2018). Superoxide dismutases: Dual roles in controlling ROS damage and regulating ROS signalling. The Journal of Cell Biology, 217(6): 1915–1928. https://doi.org/10.1083/jcb.201708007.
  51. Wolff, S. P. (1994). Ferrous ion oxidation in presence of ferric ion indicator xylenol orange for measurement of hydroperoxides. Methods in Enzymology. 233: 182-189.
  52. Xia, Y. and Zweier, J. L. (1997). Measurement of myeloperoxidase in leukocyte-containing tissues. Analytical Biochemistry, 245(1): 93–96. https://doi.org/10.1006/abio.1996.9940
  53. Zhang, H., Butters, T., Adeniran, I., Higham, J., Holden, A. V., Boyett, M. R. and Hancox, J. C. (2012). Modeling the chronotropic effect of isoprenaline on rabbit sinoatrial node. Frontiers in Physiology, 3: 241. https://doi.org/10.3389/fphys.2012.00241
  54. Zhou, R., Xu, Q., Zheng, P., Yan, L., Zheng, J. and Dai, G. (2008). Cardioprotective effect of fluvastatin on isoprenaline-induced myocardial infarction in rat. European Journal of Pharmacology, 586(1-3): 244–250. https://doi.org/10.1016/j.ejphar.2008.02.057
  55. Zitron, E., Scholz, E., Owen, R. W., Lück, S., Kiesecker, C., Thomas, D., Kathöfer, S., Niroomand, F., Kiehn, J., Kreye, V. A., Katus, H. A., Schoels, W. and Karle, C. A. (2005). QTc prolongation by grapefruit juice and its potential pharmacological basis: HERG channel blockade by flavonoids. Circulation, 111(7): 835–838. https://doi.org/10.1161/01.CIR.0000155617.54749.09

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